I read a book recently in which a sperm sample had been sent to a lab and I believe they had a sample of some sort of bodily fluid from a grand father of a family so the lab was able to deduce that the sperm sample came from one of the lads in that particular family. At this point I should say taht the book in question I was reading was a scandinavian murder mystery so I have no idea if my answer is anything even remotely resembling a fact! :c)
There was a similar plot in the radio 4 soap opera "The archers" - some years ago. Each of 2 brothers claimed to be the father of a boy, but DNA analysis did distinguish which of the 2 brothers was the real father of the child.
Each parent contributes 50 percent of a child's DNA, but never the same 50 percent to any 2 children.