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No best answer has yet been selected by the freak. Once a best answer has been selected, it will be shown here.
For more on marking an answer as the "Best Answer", please visit our FAQ.If it is an electric guitar, you plug it into the guitar like you would the amplifier.
If it is an accoustic guitar, most of the tuners have microphones within them, you play the note and it'll tell you whether the note is sharp or flat.
Alternatively, tuning pipes; play the note on your guitar and compare it to the note produced by blowing into the relative pipe, then by ear determine the need for retuning the string.