Purifying Organelles from the cytoskeleton?

[BettsBiology]

Particularly lysosomes. I think I'm getting a low yield as some remain attached to the cytoskeleton and are then lost. I want to add something that could dissociate the lysosomes from the cytoskeleton. I have "isocortisol" written down in my notes, but I can't seem to find information about it online...
Have I misheard/spelt it wrong? Or is there something else I can use?

Thanks!

[theprof]

In very general terms, Percoll density gradient centrifugation will do the job and there are methods of enhancing the results if necessary. Certain tissues require other methods such as isopycnic centrifugation in a metrizamide gradient, Nycodenz gradients or Iodixanol gradients.

Yes isocortisol is used for dissociation, but before I discuss this in depth, it would be helpful to know what tissue you are using as I don't want to provide you with unnecessary additional information if you're not going to use it.

Try searching by specifying "in vitro" in your search parameters. You may get better results.

What course of study are you undertaking?

[theprof]

Ah well, it's been a week now since I replied. The question was reasonably complex, the answer, out of necessity, even more so.

I wonder if BettsBiology has reconsidered if this is the career path he or she wishes to follow.