time spare
I think I will try to describe the new test amidst all the awful crap I am reading - - ( triggered by the tele Scientist saying - "fish" )
the machine looks like those binocular toy things wivva clicker and may put the specimen to be tested froo various baths
( I am intentionally writing this in simple AB english )
specimen is RNA so the first bath is to get it back to DNA - using exciting reverse transcriptase
do the DNA strand and get it to amplify - froo cycles of PCR
this is amaxingly efficient - if one cycle leads to 8 more identical strands and you do it eight times then the number is 8 to power 8. I make this to be 100 m strands - yes a hundred mill
look at a strand of ds DNS - heres one
https://www.nature.com/scitable/topicpage/discovery-of-dna-structure-and-function-watson-397/
the two strands are nt exactly opposite each other
there is a small gap and a large gap ----- along which can lie a third strand of DNA . so synthesis before hand a seeker strand of DNA which corresponds to the bit you are searching and put a little light on top ( fluorescent tip_)
and the test specimen will now glow. - no binding third strand no glow - negative test
use light sensitive doo dah to pick up glow
postive test - all automated
and that boys and gurlz is the basis of the fluorescent in situ hybridisation test ( FISH )
see line one - where the tele scientist let slip the word 'fish'
( "that is the fish you are looking for" tele scientist delphically)
thank you for getting down here
( oh god there is some awful crap on AB tonight)