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Glucose in HPLC

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amywy88 | 17:51 Fri 04th May 2007 | Science
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Why is it difficult to analyse glucose directly using HPLC with UV detection?
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Avatar Image theprof
Well, assuming that you need another short answer, it's because glucose absorbs virtually no UV light.

21:21 Fri 04th May 2007
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is that because it has no chromophore?
22:39 Fri 04th May 2007
Avatar Image theprof
Yes, that's right. This problem occurs in the majority of saccharides.

This is also why other methods are used for other molecules lacking chromophores such as amines and aldehydes.
23:23 Fri 04th May 2007

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