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Many thanks for all your answers, The vet tried for ages and covered the majority of the pup's body but couldn't find it so I suggested that the scanner may be faulty so he got an unopened microchip and checked it with the scanner and it registered, so my wife rang the breeder to find out where her vet had put the microchip and the vet went all round the area where the chip was meant to be but still couldn't find it so he said he would check when the pup goes back again in 2 weeks for her second injection. He said she couldn't be microchipped a second time if it is still there hence the reason that he said he would check again in 2 weeks. He said the needle which they use for the microchip is quite big and leaves a hole in the skin, and on the very odd occasion the chip will fall out